NATURAL SCIENCES

Journal of fundamental
and
applied researches

THE POLYMORPHIC VARIANTS OF FOLATE CYCLE GENES IN AN ASTRAKHAN POPULATION OF THE KAZAKH AND RUSSIAN NATIONAL

2016. №3, pp. 39-43

Lomteva Natalia A. - Ph.D. (Biology), Associate Professor, Astrakhan State University, 1 Shaumiana Sq., Astrakhan, 414000, Russian Federation, molecula01@yandex.ru

Zhukova Yuliya D. - Researcher, Astrakhan State University, 1 Shaumiana Sq., Astrakhan, 414000, Russian Federation, molecula01@yandex.ru

Petryakova Yuliya A. - student, Astrakhan State University, 1 Shaumiana Sq., Astrakhan, 414000, Russian Federation, molecula01@yandex.ru

Urkumbaeva Saule K. - student, Astrakhan State University, 1 Shaumiana Sq., Astrakhan, 414000, Russian Federation, molecula01@yandex.ru

Kondratenko Elena I. - D.Sc. (Biology), Professor, Astrakhan State University, 1 Shaumiana Sq., Astrakhan, 414000, Russian Federation, molecula01@yandex.ru

In this study we shown the results of the research of gene polymorphism of folate cycle of Astrakhan volunteers of two ethnic groups: Russian and Kazakh. Folate cycle is a complex and important stage of metabolism, which occurs in the metabolism of homocysteine. Folic acid deficiency leads to various disorders in the body. The study was conducted on volunteer women 18-33 years. For the genetic study was sampled blood from a vein. DNA extraction was performed from peripheral blood using a reagent kit “Proba-Rapid Genetics”. Polymorphisms in genes was determined by PCR Realtime. The study identified the occurrence frequency of alleles and genotypes of polymorphic loci MTHFR: _677_ C > T, MTHFR: _1298_ A > C, MTR: _2756_ A > G, MTRR: _66_ A > G. According to the results from a sample of more susceptible to the appearance of homozygous nucleotide substitutions and related metabolic effects representatives of the Kazakh nationality.

Key words: фолатный цикл, полиморфные варианты генов, полиморфизм генов, предрасположенность к заболеваниям, ферменты фолатного метаболизма, этническая принадлежность, folate cycle, polymorphic variants of genes, gene polymorphism, susceptibility to disease, the enz