NATURAL SCIENCES
Journal of fundamental
and
applied researches
The study of the neuroprotective effect of the extract of the insect in vitro
2016. №1, pp. 34-44
Aldarmaa Jalsrai - Ph.D. (Biology), Senior Researcher, Institute of Traditional medicine and Technology, 214 РЎhingiss Khan Str., Ulaanbaatar, 210646, Mongolia, jaldarmaa@gmail.com
Sanzhieva L.Ts. - Ph.D. (Biology), Senior Researcher, Buryat State University, 24a Smolina Str., Ulan-Ude, 670000, Russian Federation, lts_sanzh@mail.ru
Suslov N.I. - D.Sc. (Medicine), Professor, Research Institute of Pharmacology and Regenerative Medicine, 3 Lenin Ave., Tomsk, 634028, Russian Federation, suslov_ni@pharmso.ru
Dygai A.M. - D.Sc. (Medicine), Professor, Research Institute of Pharmacology and Regenerative Medicine, 3 Lenin Ave., Tomsk, 634028, Russian Federation, dygai_am@pharmso.ru
Insects and the substances extracted from them have been used as medicinal resources by human cultures all over the world. The substances extracted from darkling beetles ( Blaps femoralis ) has been used as antimicrobial, analgesic, anti ulcer agents in Asian countries. Aim of this study was to evaluate neuroprotective effects against neurotoxicity in vitro and clarify possible mechanism. Results: Blaps femoralis extract has been demonstrated to scavenge hydroxyl radicals by ESR. Half-maximal inhibitory concentration, IC50 of the extract was 11.35 mg/ml. It restored the decreased level of complex-I, MDH activities in a concentration of 8.0, 17.0 and 35.0 mg/ml. The neuroprotective effects were assessed by HO-induced neurotoxicity in primary cultured cortical neurons. Also, pretreatment with the extract Blaps femoralis (0.5 mg/ml) was found to impart significant protection against HO-induced neurotoxicity in primary cultured cortical neurons. Furthermore, the use of a luciferase reporter (ARE-lic) assay showed that extract from Blaps femoralis activates protective signaling pathway in primary cortical neurons through ARE/Nrf2 pathway.
Key words: Blaps femoralis, насекомые, нейропротективное действие, insects, neuroprotection