CHARACTERISTICS OF THE PROLIFERATIVE ACTIVITY OF THE ANTERIOR EPITHELIUM OF THE MAMMALIAN CORNEA IN THE POSTNATAL PERIOD OF ONTOGENESIS UNDER THE ACTION OF EXTERNAL FACTORS
Sentyurova Lyudmila Georgievna - Doctor of Medical Sciences, Professor, Head. Department of Biology, Federal State Budgetary Educational Institution of Higher Education "Astrakhan State Medical University", 414000, Astrakhan Region, Astrakhan, Bakinskaya, 121, sentlj2012@yandex.ru
Kramorenko Oleg Vladimirovich - Candidate of Medical Sciences, doctor, Astrakhan State Medical University" of the Ministry of Health of the Russian Federation, 414000, Astrakhan, Baku str., 121,
Berlyakova Elena Mikhailovna - сandidate of medical sciences, associate professor, associate professor, Astrakhan State Medical University, 414000, Astrakhan, Baku Str., 121,
Mazlov Alexey Mikhailovich - Senior Lecturer, Astrakhan State Medical University, 414000, Astrakhan, Baku Str., 121,
Sherysheva Yulia Vladimirovna - Candidate of Medical Sciences, Associate Professor of the Department of Infectious Diseases and Epidemiology, Federal State Budgetary Educational Institution of Higher Education "Astrakhan State Medical University", 414000, Astrakhan Region, Astrakhan, Bakinskaya st., 121,
Morozov Vladimir Dmitrievich - 5th year student of the Faculty of Medicine, Federal State Budgetary Educational Institution of Higher Education "Astrakhan State Medical University", 414000, Astrakhan Region, Astrakhan, Bakinskaya st., 121,
The structural and functional features of the anterior epithelium of the mammalian cornea under the influence of external factors have been studied. Changes in the process of postnatal corneal development of mongrel white rats ranging from one day of age to 28 days after the action of H2S on the cornea at doses of 3.30 and 300 mg/m3 are described. It was found that a dose of 3 mg/ m3 does not have a noticeable effect on the formation of intercellular contacts, thereby allowing optimal functioning. At this age, there is no violation of the parameters of the circadian rhythm: mesor and amplitude. As the dose of H2S increases to 30 mg/m3, the density of contact with the basement membrane suffers. And chromatin condensation is clearly visible in the nucleus. The amplitude of the circadian rhythm changes. At the action of 300 mg/m3, deeper structural changes were found in the form of the formation of myelin-like structures. In addition, pronounced violations of the parameters of the circadian rhythm of proliferative activity in its epithelium were found in all the studied age periods with desynchronosis, a significant decrease in mesor, amplitude, and a shift in phase rhythms up to its inversion. The results show the justified use of ultramicroscopy methods and the study of the proliferative index to objectify data on the action of aggressive pollutants.
Key words: cornea, ontogenesis, rats, proliferative index of the cornea, hydrogen sulfide
