THE EFFECTS OF HYPOTHERMIA ON THE FLUORESCENT CHARACTERISTICS OF RAT ERYTHROCYTE MEMBRANE PROTEINS
Klichkhanov Nisred Kadirovich - Doctor of Biological Sciences, Professor, профессор кафедры биохимии и биофизики, Dagestan State University, Russia, 367000, Republic of Dagestan, Makhachkala, st. Magomed Gadzhiev, 43-a, klich-khan@mail.ru
Jafarova Albina Mekhyadinovna - Candidate of Biological Sciences, Associate Professor, Associate Professor, Dagestan State University, Russia, 367000, Republic of Dagestan, Makhachkala, st. Magomed Gadzhiev, 43-a, albina19764@mail.ru
A decrease in body temperature in rats to 30°C (moderate hypothermia) is accompanied by the development of oxidative stress, the severity of which depends on the duration of hypo-thermia. Oxidative modifications of proteins can contribute to significant changes in their struc-tural and dynamic properties, sensitive indicators of which can be spectral characteristics. The aim of this study was to investigate the effects of hypothermia on the fluorescent characteristics of rat erythrocyte membrane proteins. It was found that short-term hypothermia leads to a significant decrease in the total fluorescence of membrane proteins. Moreover, the most significant contribution to this decrease is made by tryptophan residues. Prolongation of hypothermia con-tributes to a further decrease in both total and tryptophan fluorescence of proteins. Significant changes during hypothermia also affect the second derivatives of the spectra, which suggest sig-nificant changes in the localization of aromatic fluorophores in erythrocyte membrane proteins. It is interesting that short-term hypothermia is accompanied by a small flash of tyrosine fluores-cence, absent in the control spectra, which persists even after prolongation of hypothermia. The data obtained indicate that the most pronounced structural-conformational changes in the proteins of erythrocyte membranes occur at the initial stages of a decrease in body temperature of rats.
Key words: hypothermia, rats, erythrocytes, membranes, proteins, fluorescent characteristics
