DEPENDING THE LEVEL OF FREE-RADICAL HOMEOSTASIS OF BLOOD PLASMA IN MALE RATS ON THE DURATION OF PRENATAL STRESS

Kuleshova Olga N.  -  Ph. D. (Biology), Senior Researcher, Astrakhan State University, 1 Shaumyana Sq., Astrakhan, 414000, Russian Federation, pozdniakova_olga@list.ru

The effects of immobilization prenatal stress of different duration on free radical homeostasis of blood plasma were studied. The study was conducted on sexually mature mongrel white male rats, whose mothers were subjected to immobilization stress 1, 2 and 3 hours a day from the 16th to the 19th days of pregnancy. In the blood plasma, the level of POL, OMB, AOA, CP, and SOD activity were examined. It was found that in the plasma of male rats that underwent 1-hour prenatal immobilization, an increase in the percentage of total antioxidant activity was noted (p<0.05). 2-hour prenatal immobilization led to an increase in the level of intermediate products of lipid oxidation (p<0.01) and AOA (p<0.05). 3-hour prenatal immobilization led to a change in all the considered parameters characterizing oxidative homeostasis: an increase in the level of intermediate (p<0.01) and final products of POL (p<0.05), an increase in the level of products of free radical modification of proteins (p<0.05), an increase in total antioxidant activity (p<0.01 ) and CPU (p<0.01), decrease in SOD activity (p<0.01). Thus, the level of shifts in CP homeostasis in the blood plasma of prenatally stressed male rats directly depends on the duration of prenatal exposure to animals, and the most sensitive parameter, of those considered by us, is the level of total AOA.

Key words: oxidative modification of proteins, superoxide dismutase, free radical homeostasis, antioxidant activity, prenatal stress, male rats, plasma