REGULARITIES OF CHANGES OF NUMBER AND SIZES OF ADRENALINE GRANULES ON ERYTHROCYTES AT BLOCKADE AND STIMULATION OF ADRENERGIC REGULATORY MECHANISMS
Tryasuchev Andrey Valerievich - PhD in Biological sciences, assistant professor of the Department of Physiology, Morphology, Genetics and Biomedicine, Astrakhan State University named by V.N. Tatischev, Russia, 414000, Astrakhan, Shaumyan Sq.,1, fyzevk@rambler.ru
Tryasuchev Andrey Valerevich - Ph.D of Biological sciences, Associate Professor of the Department of Physiology, Morphology, Genetics and Biomedicine, Astrakhan State University, Shaumyana Sq., 1, Astrakhan, 414000, Russian Federation, tryandval@mail.ru
The aim to study the patterns of changes in the number and size of catecholamine granules on erythrocytes during blockade and stimulation of adrenergic regulatory mechanisms in laboratory rats. Material and methods. The number of adrenaline granules (hereafter AdrGN) on erythrocytes was determined by impregnating blood smears of intact rats with silver nitrate, with the introduction of anapriline β-adrenergic blocker (2 mg / kg) and acute stress. Results. AdrGN in rats is normally 145–155 pieces / 40 erythrocytes, sizes from 0.6 to 2.0 βm, of which almost half are medium-sized granules. After the administration of a β-adrenergic receptor blocker, the total AdrGN decreases almost threefold due to a particularly strong decrease in the number of large and medium-sized granules, the proportion of which in the total AdrGN decreases with a slight increase in the proportion of small granules. In acute stress, the AdrGN increases by almost 2 times due the number of small granules, the proportion of which sharply increases with a significant reduction in the proportion of large granules in the total AdrGN. Conclusion. The cytological method quite clearly reveals the patterns of changes in the number and size of granules on erythrocytes, which allows us to speak about the quantitative and qualitative features of the erythrocyte’s binding of catecholamines with changes in the activity of adrenergic regulation mechanisms.
Key words: adrenaline granules, erythrocytes, β-adrenergic receptor blocker, stress
