NATURAL SCIENCES

Journal of fundamental
and
applied researches

Neurometabolites influence on the level of apoptosis and behavioral activity of HER2/neu transgenic mice in aging

2015. №3, pp. 48-55

Kozlova Yulya O. - postgraduate, Sechenov Institute of Evolutionary Physiology and Biochemistry, 44 ThorezР° Ave., St. Petersburg, 194223, Russian Federation, yulya.kozlova@mail.ru

Bazhanova Elena D. - D.Sc. (Biology), Leading Researcher, Sechenov Institute of Evolutionary Physiology and Biochemistry, 44 ThorezР° Ave., St. Petersburg, 194223, Russian Federation, bazhanovae@mail.ru

Teply David L. - D.Sc. (Biology), Professor, Astrakhan State University, 1 Shaumyana Sq., Astrakhan, 414000, Russian Federation, dima.tepliy@yandex.ru

Zhuravleva Galina F. - D.Sc. (Medecine), Professor, Astrakhan State University, 1 Shaumyana Sq., Astrakhan, 414000, Russian Federation, astbio@aspu.ru

Recently, relationship established between apoptosis and aging. Apoptosis promotes to conservation tissue homeostasis by removing of damaged cells, including neurons. The loss of neurons in brain structures leads to the loss of functions in aging. There are pharmacological corrections of age-relation damages of brain, but the mechanism of some medicine action is unclear yet. So, the aim of the study is investigation of piracetam and cytoflavin influence on the level of neuron apoptosis, psychoemotional state and motion activity of transgenic mice HER2/neu in aging. We detected the apoptosis level by TUNEL, CD95 expression by immunohistochemistry. We estimated behavior and locomotion by “an open field” test and Suok test. The results showed that the piracetam and cytoflavin have neuroprotective and anxiolytic action, improved brain activity. Besides, these medicines can be involved in apoptosis regulation, and this fact should be considered in practical therapy.

Key words: старение, нейроны, кора головного мозга, апоптоз, поверхностный рецептор CD95, трансгенные мыши HER2/neu, пирацетам, цитофлавин, двигательная активность, тревожность, aging, neurons, cortex, apoptosis, receptor CD95, HER2/neu transgenic mice, piracetam, c