NATURAL SCIENCES
Journal of fundamental
and
applied researches
The features of human viruses research by high-throughput sequencing methods
2014. №4, pp. 100-108
Rubalskiy Evgeniy O. - post-graduate student, G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 10 Admiral Makarov Str., Moscow, 125212, Russian Federation, info@gabrich.com
Tikhonova Nina T. - Sc.D. (Biology), Professor, Head of the Laboratory, G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 10 Admiral Makarov Str., Moscow, 125212, Russian Federation, info@gabrich.com
Aleshkin Vladimir A. - D.Sc. (Biology), Professor, Director, G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 10 Admiral Makarov Str., Moscow, 125212, Russian Federation, info@gabrich.com
Chikobava Merab G. - Academic Doctor Ph.D., Associate Professor, Sukhumi State University, 12 Anna Politkovskaia Str., Tbilisi, 0186, Georgia, merab_ch@mail.ru
Afanasyev Stanislav S. - D.Sc. (Medicine), Professor, Deputy Director, G.N. Gabrichevsky Research Institute for Epidemiology and Microbiology, 10 Admiral Makarov Str., Moscow, 125212, Russian Federation, info@gabrich.com
Rubalskaia Alvina F. - post-graduate student, Astrakhan State Medical University, 121 Bakinskaya Str., Astrakhan, 414000, Russian Federation, rubalsky.innovation@gmail.com
In the present review examines the use of modern high-throughput sequencing methods (next generation sequencing, NGS) to study human viruses. The NGS features, imposed by the set of practical problems at de-novo whole genome sequencing, resequencing, RNA sequencing metagenomic sequencing are analyzed and summarized. The NGS approaches are presented in accordance with the key issues that are important for the effective use of high-throughput sequencing methods. Some features of the sample preparation, isolation of nucleic acids, whole genome amplification, amplicons producing, libraries preparation for sequencing are shown. The typical steps of the NGS data bioinformatics analysis are presented considering the applications of medical virology.
Key words: высокопроизводительное секвенирование, NGS, вирусы человека, полногеномное секвенирование, секвенирование de-novo, секвенирование РНК, метагеномное секвенирование, биоинформационный анализ, high-throughput sequencing, NGS, human viruses, whole genome sequ