NATURAL SCIENCES

Journal of fundamental
and
applied researches

The karyological characteristic of Lophanthus anisatus Benth.

2013. №2, pp. 86-98

Kozak Margarita F.  - Sc.D. (Biology), Professor, Astrakhan State University, 1 Shaumian Sq., Astrakhan, 414000, Russian Federation, mkozak@yandex.ru

Turdugulova Raushan T.  - post-graduate student, Astrakhan State University, 1Shaumian Sq., Astrakhan, 414000, Russian Federation, mkozak@yandex.ru

Lophanthus anisatus Benth. an perennial grassy essential oil plants of family Lamiaceae. The purpose of work was the analysis of a karyotype, morphometric parameters of chromosomes, their identification, drawing up the ideogram of chromosomes. Studying of the karyotype was carried out in cages of an apically meristem of backs on metaphase plates, on temporary preparations with use of preprocessing by colchicines for shortening of chromosomes and identification of a centromeric zone. Coloring of backs carried out aсetoсarminе by the original technique developed on the basis of the standard. The structure of the karyotype Lophanthus anisatus Benth introduced in the Astrakhan region was studied. Results of the morphometric analysis of chromosomes are presented. The set of chromosomes (2n=18) contains 7 couples (I, II, IV–VII, IX) submetacentric and 2 couples (III, VIII) metacentric chromosomes. This look belongs to group of types of the Lamiaceae family having in a diploidic set of 18 chromosomes. The average total absolute length of a set chromosomes is equal 41, 6±1,85 mcm (C.V. 14,2 %). Coefficients of a variation of chromosomal indicators correspond to average and high levels of variability. By means of computer system of VideoTest-Kario-3.0 chromosomes are identified; the formula of the karyotype and the ideogram of chromosomes was made.

Key words: Lophanthus anisatus,Family Lamiaceae,karyotype,karyotype formula,ideogram,identification of chromosomes,morphometric parameters of the chromosomes,introduction