NATURAL SCIENCES
Journal of fundamental
and
applied researches
The molecular-genetic identification of Russian sturgeon(Acipenser gueldenstaedtii) from natural populationsin the Volga-Caspian basin
2013. ¹2, pp. 82-86
Bazelyuk Nadezhda N. - Ph.D. (Philosophy), Chief of laboratory, Caspian Fisheries Research Institute, 1 Savushkin St., Astrakhan, 414056, Russian Federation, bazelyuk@yandex.ru
Natalya V. Kozlova - Ph.D. (Biology), Research Assistant, Caspian Fisheries Research Institute’, 1 Savushkin St., Astrakhan, 414056, Russian Federation, kaspiy-info@mail.ru
Mukhamedova Rufiya M. - junior research assistant, Caspian Fisheries Research Institute, 1 Savushkin St, Astrakhan, 414056, Russian Federation, kaspiy-info@mail.ru
The relevance of molecular-genetic studies and identification of Russian sturgeon is predetermined by wide variation of their phenotype caused by environmental factors. The application of the methods of molecular-genetic identification of sturgeons from natural populations of the Volga-Caspian basin will help to identify their population and species level and to assess genetic diversity in order to preserve the nuclear stock of valuable fish species. The scientists from the laboratory of fish physiology and genetics of the scientific and experimental complex for molecular and genetic investigations of FSUE “CaspNIRKh” have analyzed the intraspecies variability of mitochondrial DNA element and nuclear DNA element, extracted from tissue specimens (fragments of fins) of 87 specimens of Russian sturgeon, caught at the Volga river and at the Russian part of the Northern Caspian. Three species-specific primers were used for specific identification of two mitochondrial mitotypes by PCR method. Four locuses with fluorescent marks were used for microsatellite analysis of nuclear DNA fragments. The considered methods of the molecular-genetic identification of sturgeons are reliable for species identification both of specimen and eggs and other processing products.
Key words: sturgeon,mitochondrial DNA,nuclear DNA,microsatellite analysis,mitotypes,polymorphism,polymerase chain reaction,primers,amplification