NATURAL SCIENCES

Journal of fundamental
and
applied researches

Selection of the temperature regimes of freezing during cryopreservation of inconnu oocytes

2017. №4, pp. 182-185

Firsova A.V. - postgraduate student, Astrakhan State Technical University, 16 Tatisheva Str., Astrakhan, 414056, Russian Federation, firsovaangelina1991@mail.ru

Preservation of valuable fish species genetic diversity is one of the main aims of modern aquaculture. At present, the use of low temperature preservation methods remains one of the most attractive and fast-growing ways of rare and endangered species conservation. The method of deep freezing of valuable fish sperm is applied for gene pool preserving today. However, the issue of eggs preservation doesn’t have solution. The importance of cryopreservation of cells has a rate of decrease in temperature. The aim of this work was to determine the optimum temperature of freezing during cryopreservation of inconnu oocytes. The material of investigation was inconnu eggs. The effect on the eggs of inconnu from the two modes of freezing - fast and slow was studied. 33% of oocytes after thawing had a damaged shell when using the method of ultrafast freezing. Thawing eggs, frosting by the slow freezing showed that eggs externally whole, only a few eggs with a damaged shell. In both cases, the eggs have an internal adjustment. As a result of studies it was found that cryopreservation of inconnu eggs by the method of slow freezing allows you to obtain a greater number of intact oocytes after thawing.

Key words: сохранение генофонда, яйцеклетка, половые продукты рыб, криопротектор, белорыбица, криоконсервация, режим замораживания, gene pool conservation, oocytes, sexual products of fishes, cryoprotectors, inconnu, cryopreservation, regime of freezing